文献类型：期刊文献

中文题名：气升式生物反应器在杂交瘤细胞培养中的应用

英文题名：Application of the Airlift Bioreactor for Hybridoma Cell Cultures

作者：谭文松[1];陆健[1];张元兴[1]

机构：[1]华东理工大学生化工程研究所,上海200237

年份：1996

卷号：12

期号：4

起止页码：477

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；PubMed;

基金：上海市科学技术发展基金;"八五"国家科技攻关计划

语种：中文

中文关键词：生物反应器;杂交瘤细胞;细胞培养;生物制品

外文关键词：Airlift bioreactor, hybridoma cells, cell cultures

摘要：在前述研究工作基础上,设计开发了10L规模的动物细胞培养用气升式生物反应器。应用该生物反应器悬浮培养杂交瘤细胞,通过平行试验,考察了该反应器设计的合理性和可靠性。结果显示该反应器不存在限制细胞生长、代谢和产物生成的因素,而且细胞破损被彻底消除,表明该气升式生物反应器给细胞生长、代谢和产物生成提供了理想的培养环境,其设计是成功的。
On the basis of the previous work, a 10 liter scale airlift bioreactor for animal cell cultures has been designed and developed. Here, the experimental results of the hy-bridoma cells cultured in suspension in this bioreactor have been reported. By the tests run in parallel, the reasonableness and reliability of the airlift bioreactor design was investigated at the same time compared with the performance of the CelliGen bioreactor system. The experimental results show that cell damage usually found in sparged biore-actors is prevented completely in this airlift bioreactor, and there are no factors present in it to limit the cell growth. This indicates that the culture environment provided by this airlift bioreactor for animal cell growth, metabolism and product formation is ideal. Therefore, it can be concluded that the design of this airlift bioreactor is successful.