文献类型：期刊文献

英文题名：Biosynthesis of (R)-3-hydroxybutyric acid from syngas-derived acetate in engineered Escherichia coli

作者：Fei, Peng[1];Luo, Yuanchan[1];Lai, Ningyu[1];Wu, Hui[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：336

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20212210445894);WOS:【SCI-EXPANDED(收录号:WOS:000672807800001)】；

基金：This study was supported by the Fok YingTong Education Foundation, China (Grant No. 161017) , the National Natural Science Foundation of China (Grant No. 21776083) , the Natural Science Foundation of Shanghai (19ZR1472700) , the Fundamental Research Funds for the Central Universities (Grant No. 22221818014) , the 111 Project (B18022) . Partially supported by Open Funding Project of the CAS Key Laboratory of Synthetic Biology. We thank Professor Yang Gu from CASKey Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences for kindly providing us the syngasderived acetate produced by Clostridium ljungdahli.

语种：英文

外文关键词：Syngas-derived acetate; (R)-3-hydroxybutyric acid; propionyl-CoA transferase; Clostridium beijerinckii; Escherichia coli

摘要：Acetate is a potential non-food carbon source for industrial production, coping with the shortage of food-based feedstocks. (R)-3-hydroxybutyric acid (R-3HB) can be used as an important chiral intermediate in the fine chemical and pharmaceutical industry. In this study, the R-3HB biosynthesis pathway was successfully constructed when genes of beta-ketothiolase (phaA), acetoacetyl-CoA reductase (phaB) from Ralstonia eutropha, and propionyl-CoA transferases (pct) from Clostridium beijerinckii 8052 were introduced into Escherichia coli. The effects of host E. coli strains, different propionyl-CoA transferases, and post-induction temperatures were investigated. The final concentration of R-3HB reached 0.86 g/L using acetate as the sole carbon source. Subsequently, a kind of culture broth containing the syngas-derived acetate was used to produce 1.02 g/L of R-3HB with a yield of 0.26 g/g. In this study, the engineered E. coli strain could efficiently utilize syngas-derived acetate to synthesize R-3HB.