文献类型：期刊文献

英文题名：A novel trichosanthin fusion protein with increased cytotoxicity to tumor cells

作者：Lin, Bing[1];Yang, Xu-Zhong[2];Cao, Xue-Wei[1];Zhang, Tao-Zhu[2];Wang, Fu-Jun[2,3];Zhao, Jian[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Zhejiang Reachall Pharmaceut Co Ltd, Dongyang 322100, Zhejiang, Peoples R China;[3]Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

年份：2017

卷号：39

期号：1

起止页码：71

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20164202899877);WOS:【SCI-EXPANDED(收录号:WOS:000391452600009)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 81571795). The authors alone are responsible for the content and writing of this article.

语种：英文

外文关键词：Antitumor activity; Apoptosis; Drug delivery; Heparin-binding EGF-like growth factor; Trichosanthin

摘要：To evaluate the anti-tumor effects of trichosanthin after fusion with a cell penetrating peptide, heparin-binding peptide (HBP), derived from human heparin-binding EGF-like growth factor (HB-EGF). The fusion protein of trichosanthin-HBP was expressed in Escherichia coli BL21 and purified by Ni-NTA affinity chromatography. The HBP domain had no influence on the topological inactivation activity and N-glycosidase activity of trichosanthin. Trichosanthin-HBP significantly inhibited the growth of tested cancer cells which are impervious to trichosanthin. Tumor cell apoptosis and both the mitochondrial- and death receptor-mediated apoptotic signaling pathways induced by trichosanthin-HBP were more significant than those induced by trichosanthin in HeLa cells. HBP is an efficient intracellular delivery vehicle for trichosanthin and makes trichosanthin-HBP become a promising agent for cancer therapy.