文献类型：期刊文献

中文题名：反应条件对脂肪酶酯化活性和对映选择性的影响

英文题名：Effect of Reaction Parameters on Reactivity and Enantioselectivity of Lipase-catalyzed Esterification

作者：刘幽燕[1];许建和[1];刘洪来[2];胡英[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]华东理工大学化学系,上海200237

年份：1999

卷号：13

期号：4

起止页码：372

中文期刊名：高校化学工程学报

外文期刊名：Journal of Chemical Engineering of Chinese Universities

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金!29506043

语种：中文

中文关键词：脂肪酶;对映选择性;萘普生;酯化反应;萘基丙酸

外文关键词：lipase; reactivity; enantioselectivity; naproxen; esterification

摘要：分别测定了2-萘基丙酸类药物萘普生R-、S-对映体在有机溶剂中的酶促酯化反应初速度，并研究了反应温度、酰基受体和有机溶剂等反应条件对脂肪酶的反应活性和对映选择性的影响。在一定范围内，酶反应活性随温度升高而增加，温度过高易使酶失活；酶选择性则随温度升高而下降；反应温度以35～40℃为佳。在疏水性强的溶剂中，酶反应活性较大；酶的选择性则以异辛烷、苯、甲苯等为溶剂时较好。合适的混合溶剂系统能使酶表现出比在单一溶剂中更好的活性和选择性；这与外消旋萘普生的实际酶促拆分结果是一致的。
The initial reaction rates of S- and R-naproxen esterification catalyzed by lipase wereseparately measured to characterize the reactivity and enantioselectivity of the enzyme and the effect ofreaction parameters including temperature, acyl receptor and solvent have been investigated.Increasing temperature, reactivity increased while enantioselectivity decreased,with an optimumbetween 35-40℃. The enzyme showed higher activity in hydrophobic solvents (with high LogP value)such as isooctane, cyclohexane. Higher enantioselectivity was obtained in isooctane, benzene, andtoluene. Higher reactivity and enantioselectivity were observed in an appropriate mixed solvent system(benzene + cyclohexne) than in the single solvent, which is consistent with the result of enzymaticresolution of racendc naproxen.