文献类型：期刊文献

英文题名：Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol

作者：Xiong, Zhi-Qiang[1]; Guo, Mei-Jin[1]; Guo, Yuan-Xin[1]; Chu, Ju[1]; Zhuang, Ying-Ping[1]; Zhang, Si-Liang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China

年份：2009

卷号：100

期号：2

起止页码：1011

外文期刊名：Bioresource Technology

收录：EI(收录号：20084211646557)

语种：英文

外文关键词：Extraction - Ethanol - Fermentation - Energy utilization - Peptides

摘要：Reduced glutathione (GSH) from fermentation broth of Saccharomyces cerevisiae was extracted with ethanol without disruption of the cells. The effects of ethanol concentration, extraction temperature and extraction time were assessed by using 23 full factorial designs (FFD). Preliminary studies showed that ethanol concentration had the most influence on GSH yield by ethanol extraction, based on the first order regression coefficients derived using MINITAB software, and an optimal ethanol concentration (25%, v/v) was obtained. However, compared to the conventional extraction technique (hot water extraction), there was no significant advantage in yield of GSH from yeast cells using ethanol extraction under these optimized conditions. But ethanol extraction has several advantages, such as lower energy consumption and lower protein concentration of extraction broth, which may reduce the complexity and cost of the purification process. Hence, ethanol extraction which does not disrupt yeast cells could be an inexpensive, simple and efficient alternative to conventional extraction techniques in the GSH industry. ? 2008 Elsevier Ltd. All rights reserved.