文献类型：期刊文献

英文题名：Chemically gated artificial nanochannels for programmable subcellular signal modulated transport regulation

作者：Wu, Man-Sha[1,2,3,4];Du, Xi-Chen[1,2,3,4];Zhou, Ze-Rui[5];Wang, Xiao-Yuan[1,2,3,4];Zheng, Shi-Yu[1,2,3,4];Lv, Jian[6];Chen, Bin-Bin[1,2,3,4];Li, Da-Wei[1,2,3,4];Qian, Ruo-Can[1,2,3,4]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Lab Precis Chem, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai, Peoples R China;[5]Univ Texas Austin, Dept Chem, Austin, TX USA;[6]Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai, Peoples R China

年份：2025

卷号：16

期号：1

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001651213800009)】；

基金：This research was supported by the National Natural Science Foundation of China (22176058 to D.W.L.), the Science and Technology Commission of Shanghai Municipality (23ZR1416100 to R.C.Q., 24DX1400200 to R.C.Q.), the Program of Introducing Talents of Discipline to Universities (B16017), and the Fundamental Research Funds for the Central Universities (222201717003). The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for their help with characterizations. The authors thank Eceshi (www.eceshi.com) for the TEM analysis.

语种：英文

摘要：Biomimetic artificial nanochannels have been developed rapidly because of their promising potentials in biomedical applications. Here we report the design of chemically gated artificial nanochannels to perform transmembrane channel-mimetic permeability transition via the multi-functional DNA components modified at the inner surface, whereby the structure and charge of the DNA components can be tuned by multiple key chemical signals. We realize the targeted capture of a single mitochondrion in single living cells, which allows in situ response of multiple mitochondrial signals (Ca2+ / ROS / H+) and the subsequent delicate control of permeability transition. Further study of rotenone (ROT) induced ROS / Ca2+ release and mitochondrial membrane potential loss demonstrate that the nanochannels can response to complex chemical signals at a localized subcellular region in spite of the complicated intracellular environment. Finally, we report the advanced applications of nanochannels for evaluating and regulating the interaction network between mitochondria and other organelles.