文献类型：期刊文献

英文题名：Moracin C, A Phenolic Compound Isolated from Artocarpus heterophyllus, Suppresses Lipopolysaccharide-Activated Inflammatory Responses in Murine Raw264.7 Macrophages

作者：Yao, Xue[1];Wu, Dang[1];Dong, Ningning[1];Ouyang, Ping[1];Pu, Jiaqian[1];Hu, Qian[1];Wang, Jingyuan[1];Lu, Weiqiang[2,3];Huang, Jin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, 500 Dongchuan Rd, Shanghai 200241, Peoples R China;[3]East China Normal Univ, Sch Life Sci, 500 Dongchuan Rd, Shanghai 200241, Peoples R China

年份：2016

卷号：17

期号：8

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000382337900013)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (grants 81402482), the Shanghai Committee of Science and Technology (grants 14ZR1411100, 15431902000), China Postdoctoral Science Foundation grant (2014M551361, 2015T80415).

语种：英文

外文关键词：inflammation; MAPKs; Moracin C; NF-kappa B; phenols

摘要：Artocarpus heterophyllus, a popular tropical fruit commonly known as the jackfruit tree, is normally planted in subtropical or tropical areas. Since a variety of phytochemicals isolated from A. heterophyllus have been found to possess potently anti-inflammatory, antiviral and antimalarial activities, researchers have devoted much interest to its potential pharmaceutical value. However, the exact mechanism underlying its anti-inflammatory activity is not well characterized. In this study, seven natural products isolated from A. heterophyllus, including 25-Hydroxycycloart-23-en-3-one (HY), Artocarpin (AR), Dadahol A (DA), Morachalcone A (MA), Artoheterophyllin B (AB), Cycloheterophyllin (CY) and Moracin C (MC) were collected. Lipopolysaccharide (LPS)-stimulated inflammatory response in RAW264.7 macrophages were used in this study. Among these compounds, MC significantly inhibited LPS-activated reactive oxygen species (ROS) and nitric oxide (NO) release without marked cytotoxicity. Furthermore, MC effectively reduced LPS stimulated up-regulation of mRNA and protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and serval pro-inflammatory cytokines (interleukin-1 fi (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor ff (TNF-ff)). Mechanistic studies revealed that the anti-inflammatory effect of MC was associated with the activation of the mitogen activated protein kinases (MAPKs) (including p38, ERK and JNK) and nuclear factor-kappa B (NF-kappa B) pathways, especially reducing the nuclear translocation of NF-kappa B p65 subunit as revealed by nuclear separation experiment and confocal microscopy.