文献类型：期刊文献

英文题名：Artificial Multienzyme Supramolecular Device: Highly Ordered Self-Assembly of Oligomeric Enzymes In Vitro and In Vivo

作者：Gao, Xin[1];Yang, Shuai[1];Zhao, Chengcheng[1];Ren, Yuhong[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：53

期号：51

起止页码：14027

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20145100340157);WOS:【SCI-EXPANDED(收录号:WOS:000346484400008)】；

基金：This work was funded by the National Special Fund for the State Key Laboratory of Bioreactor Engineering (2060204), the National Natural Science Foundation of China (No. 21076079), the National major science and technology projects of China (No. 2012ZX09304009), and the Fundamental Research Funds for the Central Universities of China.

语种：英文

外文关键词：biocatalysis; enzyme catalysis; oligomerization; self-assembly; supramolecular chemistry

摘要：A strategy for scaffold-free self-assembly of multiple oligomeric enzymes was developed by exploiting enzyme oligomerization and protein-protein interaction properties, and was tested both in vitro and in vivo. Octameric leucine dehydrogenase and dimeric formate dehydrogenase were fused to a PDZ (PSD95/Dlg1/zo-1) domain and its ligand, respectively. The fusion proteins self-assembled into extended supramolecular interaction networks. Scanning-electron and atomic-force microscopy showed that the assemblies assumed two-dimensional layer-like structures. A fluorescence complementation assay indicated that the assemblies were localized to the poles of cells. Moreover, both in vitro and in vivo assemblies showed higher NAD(H) recycling efficiency and structural stability than did unassembled structures when applied to a coenzyme recycling system. This work provides a novel method for developing artificial multienzyme supramolecular devices and for compartmentalizing metabolic enzyme cascades in living cells.