文献类型：期刊文献

英文题名：Regulation mechanism of virulence by environmental acidic stress mediated by Prc in Edwardsiella piscicida

作者：Ahmed, Moamer A. H.[1];Ma, Jiabao[1];Shao, Shuai[1,4];Wang, Qiyao[1,4];Xu, Rongjing[3];Yin, Kaiyu[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Business Sch, Sch Hospitality Management, Shanghai 200235, Peoples R China;[3]Yantai Tianyuan Aquat Co Ltd, Yantai, Shandong, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2023

卷号：565

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000907803100006)】；

基金：This work was supported by grants from National Natural Science Foundation of China (32102852 to YKY) and Agriculture Research System of China of MOF and MARA (CARS -47) . All animal protocols used in this study were approved by the Animal Care Committee of the East China University of Science and Technology (2006272) . The Experimental Animal Care and Use Guidelines from Ministry of Science and Technology of China (MOST -2011-02) were strictly followed.

语种：英文

外文关键词：Edwardsiella piscicida; Prc protease; Acid stress; Virulence; In vivo colonization

摘要：Edwardsiella piscicida is a conditional pathogen that causes devastating infections in numerous fish species, leading to huge economic losses in the aquaculture industry. The E. piscicida pathogenicity is majorly attributable to its type III and VI secretion systems (T3/T6SSs) that are regulated by a two-component system (TCS) EsrA-EsrB. In this study, we illustrated that a PDZ-domain-containing protease (Prc) regulated the expression of esrB and T3/T6SSs in different manners at different growth stages. Furthermore, Prc mediated the regulation of environmental acidic signals on the expression of esrB. Mutation of esrA impaired E. piscicida virulence expression irrespective of the ambient acidic signals. Collectively, Prc could react with the activated EsrA and influence the expression of esrB and T3/T6SSs. These findings reveal an extensive understanding of the virulence regulation upon sensing the environmental acidic signals in bacterial pathogens.