文献类型：期刊文献

英文题名：Biofilm Disruption Utilizing α/β Chimeric Polypeptide Molecular Brushes

作者：Zhang, Si[1];Xiao, Xi-Mian[1];Qi, Fan[1];Ma, Peng-Cheng[1];Zhang, Wei-Wei[1];Dai, Cheng-Zhi[1];Zhang, Dan-Feng[1];Liu, Run-Hui[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Key Lab Ultrafine Mat, Minist Educ,Res Ctr Biomed Mat,Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：37

期号：11

起止页码：1105

外文期刊名：CHINESE JOURNAL OF POLYMER SCIENCE

收录：;EI(收录号：20212810628974);WOS:【SCI-EXPANDED(收录号:WOS:000489050800008)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 21574038, 21774031, and 21861162010), the Natural Science Foundation of Shanghai (No. 18ZR1410300), the "Eastern Scholar Professorship" from Shanghai local government (No. TP2014034), the national special fund for State Key Laboratory of Bioreactor Engineering (No. 2060204), and the Fundamental Research Funds for the Central Universities (No. 22221818014).

语种：英文

外文关键词：Molecular brush; Chimeric polypeptide; Biofilm; Antimicrobial resistance; Host defense peptide

摘要：Gram-negative bacteria can cause serious infections and are well known problems in biomedical practices. Biofilms of gram-negative bacteria are notorious for their frequently encountered resistance toward antibiotics. We demonstrate that alpha/beta chimeric polypeptide molecular brush (alpha/beta CPMB) exerts potent activities against antibiotic-resistant gram-negative bacteria. MTT viability assay, bacterial colony counting, and live/dead staining all indicate that alpha/beta CPMB not only inhibits biofilm formation of gram-negative Pseudomonas aeruginosa and Acinetobacter baumannii, but also effectively disrupts mature biofilms that are highly resistant to one of the most active antibiotics-colistin. The superior antibacterial performance of the alpha/beta CPMB implies its potential topical applications in treating biofilms.