文献类型：期刊文献

英文题名：DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-L-methionine production in Pichia pastoris

作者：Hu, Hui[1];Qian, Jiangchao[1];Chu, Ju[1];Wang, Yong[1];Zhuang, Yingping[1];Zhang, Siliang[1]

机构：[1]E China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol Shanghai, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：141

期号：3-4

起止页码：97

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000266672600001)】；

基金：We are grateful to National Basic Research Program (973 Program No. 2007CB714306), the National High Technology Research and Development Program of China (863 Program No.2007AA100601) and Science and Technology Commission of Shanghai Municipality (07pj14027) for their financial supports to this research.

语种：英文

外文关键词：S-adenosyl-L-methionine; Methionine adenosyltransferase; DNA shuffling; Pichia pastoris

摘要：S-Adenosyl-L-methionine (SAM) is an important molecule for normal cell function and Survival. To enhance methionine adenosyltransferase (MAT, EC2.5.1.6) activity and thus SAM production in recombinant Pichia pastoris, MAT genes from Escherichia coli, Saccharmyces cerevisiae, and Streptomyces spectabilis were recombined by DNA Shuffling. The shuffled genes were transformed into P. pastoris GS115 to construct recombinant strains and screened for high MAT activity and enhanced SAM production mutants. In the two best recombinant strains, the MAT activities were respectively 201% and 65% higher than the recombinant strains containing the starting MAT genes, and the SAM concentration increased by 103% and 65% respectively. The analysis on the deduced sequences of five representative MAT variants showed that the K18R Mutation probably resulted in the increased activity of the best MAT, which may provide new insights into structure-enzyme activity relationship of MAT and might shed light on the further rational evolution of MAT. Finally, a 6.14 gl(-1) of SAM production was reached in a 5001 bioreactor with the best recombinant Strain GS115/DS56, which showed a favorable foreground in industrial scale production Of SAM. (C) 2009 Elsevier B.V. All rights reserved.