文献类型：期刊文献

英文题名：A Three-Channel Fluorescent Probe That Distinguishes Peroxynitrite from Hypochlorite

作者：Zhang, Quanjuan[2];Zhu, Zhichuan[1];Zheng, Yongli[5];Cheng, Jiagao[2,3];Zhang, Na[6];Long, Yi-Tao[6];Zheng, Jing[1];Qian, Xuhong[2,4];Yang, Youjun[2,4]

机构：[1]E China Univ Sci & Technol, Dept Pharmaceut Sci, Sch Pharm, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, State Key Lab Met Matrix Composites, Shanghai 200240, Peoples R China;[6]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2012

卷号：134

期号：45

起止页码：18479

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20124815711283);WOS:【SCI-EXPANDED(收录号:WOS:000311192100001)】；

基金：The work was supported by the State Key Laboratory of Bioreactor Engineering via the National Special Fund (2060204) and the Open Funding Project, the Fundamental Research Funds for the Central Universities (WJ1014005), the Natural Science Foundation of Shanghai Municipality (11ZR1408800), and the National Natural Science Foundation of China (21106043). We thank Prof. Yufang Xu, Prof. Weiping Zhu, and Prof. Yongfeng Zhou for insightful discussions and Dr. Mark Lowry, Dr. Michelle A. Ivy, and Prof. Wei Wang for help with manuscript preparation.

语种：英文

外文关键词：Fluorescence - Probes

摘要：A novel fluorescent probe for peroxynitrite, PN600, was rationally designed on the basis of a unique fluorophore assembly approach. PN600 is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN600 is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN600 to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN600 is membrane permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN600 is a promising candidate for in vitro peroxynitrite imaging.