文献类型：期刊文献

英文题名：Picofluidic Electro-Osmosis Measurement of Cell Membrane Mechanical Properties

作者：Wang, Xiao-Yuan[1];Zhou, Ze-Rui[2];Gong, Li-Juan[1];Wu, Man-Sha[1];Zhang, Shi-Yi[1];Lv, Jian[3];Chen, Bin-Bin[1];Li, Da-Wei[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat,Joint Int Lab, Shanghai 200237, Peoples R China;[2]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA;[3]Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China

年份：2025

卷号：21

期号：9

外文期刊名：SMALL

收录：;EI(收录号：20250617830062);WOS:【SCI-EXPANDED(收录号:WOS:001411910900001)】；

基金：This research was supported by National Natural Science Foundation of China (21977031), Science and Technology Commission of Shanghai Municipality (2018SHZDZX03, 24DX1400200), Shanghai Science and Technology Committee (22ZR1416800, 23ZR1416100), and the Fundamental Research Funds for the Central Universities (222201717003). The authors thank the Research Center of Analysis and Test of East China University of Science and Technology for the help with the characterization. The authors also thank Prof. Fan Xia for his valuable advice and help on this work.

语种：英文

外文关键词：cell membranes; electrochemical measurement; label-free subcellular analysis; mechanical properties; nanopipette

摘要：Cells connect with their internal and external environments through plasma membranes, and the mechanical properties of cell membranes govern numerous biological events. Membrane detection techniques such as optical or magnetic tweezers have revealed mechanical strength by membrane-anchored modifications, but it remains challenging to develop label-free methods to reduce the influence of exogenous interference. Here picofluidic electro-osmosis measurement (PEOM), which enables direct and efficient sensing of cell membrane mechanical properties by using a glass nanopipette without labeling, is presented. By generating a picoliter electroosmotic fluid at the nanopipette tip, periodic cell membrane vibration modes are observed from current traces, which carry information on membrane mechanical properties to indicate its biological state. Based on characteristic peaks in the frequency domain, a theoretical framework to describe the vibration modes, which contains two ideal spring vibrator models corresponding to stretching and bending vibrations of cell membrane respectively, is developed. Notably, the PEOM strategy represents a label-free approach to reveal the mechanical properties of living cell membranes from two dimensions, which is completely different from other methods. Additionally, the exciting potential of PEOM is demonstrated for label-free observation of membrane mechanical property changes during different bioprocesses, including cytoskeletal alteration, membrane tension change, and mechanical polarization.