文献类型：期刊文献

英文题名：Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment

作者：Huang, Zhong-zi[1,2];Wang, Ping[3];Li, Hui[1];Lin, Kuang-fei[1];Lu, Zhi-yan[1];Guo, Xiao-jue[1];Liu, Yong-di[1]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, State Key Lab Biol Reactor Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Univ Engn Sci, Sch Chem Engn, Shanghai 201620, Peoples R China;[3]E China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China

年份：2014

卷号：94

起止页码：115

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20143800066733);WOS:【SCI-EXPANDED(收录号:WOS:000343345000017)】；

基金：Supported jointly by National Natural Science Foundation of China (51378208, 41273109, 41003031), Specialized Research Fund for the Doctoral Program of Higher Education (20110074130002), Program for New Century Excellent Talents in University (NCET-13-0797), Fok Ying Tung Education Foundation (141077), Shanghai Rising-Star Program (12QA1400800), Innovation Program of Shanghai Municipal Education Commission (14ZZ059), Fundamental Research Funds for the Central Universities (222201313008). We also would like to thank the anonymous referees for their helpful comments on this paper.

语种：英文

外文关键词：Phenol; Biodegradation; Moderately halophilics; Metabolic pathway; Compatible solutes

摘要：A moderately halophilic bacterial enrichment was able to degrade 120 mg/L of phenol in the presence of 1-2 M of NaCl within 3 d or 2.5-3 M of NaCl within 6 d. The optimal degradation was achieved at 1.5 M of NaCl and 350 mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment. (C) 2014 Elsevier Ltd. All rights reserved.