文献类型：期刊文献

英文题名：Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation

作者：Liu, Qi[1];Lu, Minping[1];Jin, Ci[1];Hou, Weiliang[1];Zhao, Liao[1];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：119

期号：6

起止页码：1509

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20220811704281);WOS:【SCI-EXPANDED(收录号:WOS:000759698400001)】；

基金：This study was supported by the National Natural Science Foundation of China (21978083, 31961133006).

语种：英文

外文关键词：adaptive evolution; cell structure; lignocellulose; microbial lipid; oleaginous yeast; ultra-centrifugation

摘要：Microbial lipid production from lignocellulose biomass provides an essential option for sustainable and carbon-neutral supply of future aviation fuels, biodiesel, as well as various food and nutrition products. Oleaginous yeast is the major microbial cell factory but its lipid-producing performance is far below the requirements of industrial application. Here we show an ultra-centrifugation fractionation in adaptive evolution (UCF) of Trichosporon cutaneum based on the minor cell density difference. The lightest cells with the maximum intracellular lipid content were isolated by ultra-centrifugation fractionation in the long-term adaptive evolution. Significant changes occurred in the cell morphology with a fragile cell wall wrapping and enlarged intracellular space (two orders of magnitude increase in cell size). Complete and coordinate assimilations of all nonglucose sugars derived from lignocellulose were triggered and fluxed into lipid synthesis. Genome mutations and significant transcriptional regulations of the genes responsible for cell structure were identified and experimentally confirmed. The obtained T. cutaneum MP11 cells achieved a high lipid production of wheat straw, approximately five-fold greater than that of the parental cells. The study provided an effective method for screening the high lipid-containing oleaginous yeast cells as well as the intracellular products accumulating cells in general.