文献类型：期刊文献

中文题名：表面活性素和溶菌酶在溶液中的相互作用

英文题名：Interaction between Surfactin and Lysozyme in Aqueous Solution

作者：刘静[1];邹爱华[1];牟伯中[1]

机构：[1]华东理工大学应用化学研究所,上海200237

年份：2010

卷号：73

期号：8

起止页码：705

中文期刊名：化学通报

外文期刊名：Chemistry

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：博士点基金新教师项目(200802511024);上海市技术国际合作项目(071607014)资助

语种：中文

中文关键词：表面活性素;溶菌酶;相互作用;荧光;圆二色谱

外文关键词：Surfactin; Lysozyme; Interaction; Fluorescence; CD spectra

摘要：表面活性素是一类由Bacillus subtilis HSO121产生的脂肽类生物表面活性剂,有良好的表面活性和特殊的生物活性。本文采用紫外可见透射法、荧光分光光度法和圆二色谱法研究了表面活性素和溶菌酶在溶液中的相互作用。实验表明,表面活性素与溶菌酶之间主要有静电作用、疏水作用和氢键作用,且两者之间的相互作用随着体系pH的增加而减弱。表面活性素与溶菌酶之间作用机理研究为表面活性素在膜蛋白分离以及药理应用提供了一定的理论依据。
Surfactin mainly produced by Bacillus subtilis HSO121 is an efficient biosurfactant with special surface and biological activities.Interactions between surfactin and lysozyme were investigated by UV-Vis transmittance,fluorescence emission spectra and circular dichroism measurements.The results indicated that the interaction between surfactin and lysozyme was mainly due to the electrostatic attraction,hydrophobic interaction and hydrogen bonds.Moreover,the interaction between surfactin and lysozyme was dependent on the pH value of the system and it was decreased as increasing of pH values.The study on the interaction between surfactin and lysozyme can provide the theoretical guidance for the membrane protein purification procedures and medical application of surfactin.