文献类型：期刊文献

中文题名：多壁碳纳米管修饰的DNA电化学传感器

英文题名：Multi-walled Carbon Nanotubes Modified DNA Electrochemical Sensor

作者：刘百祥[1]

机构：[1]华东理工大学物理系,上海200237

年份：2011

卷号：37

期号：3

起止页码：311

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：多壁碳纳米管;DNA电化学传感器;氧化锆;差分脉冲法

外文关键词：multi-walled carbon nanotubes; DNA electrochemical sensor; ZrO2; differential pulse voltammetry

摘要：基于DNA/ZrO2/MWCNT/GCE结构制备高灵敏度的DNA电化学传感器。室温下应用电化学方法将氧化锆多孔薄膜沉积至多壁碳纳米管修饰玻碳电极（MWCNT/GCE）上。多壁碳纳米管（MWCNT）大的比表面积、良好的电子传递性能、氧化锆的生物相容性和对DNA极好的吸附能力,能够显著提高DNA探针的固定量和DNA杂交的检测灵敏度。以亚甲基蓝（MB）为氧化还原媒介体,应用循环伏安法和差分脉冲法分别对DNA的固定与杂交进行了表征。结果表明,此法制备的电极灵敏度高,线性好,线性范围为2.15×10-7~2.15×10-10mol/L,检出限为6.58×10-12mol/L。
A DNA/ZrO2/MWCNT/GCE electrochemical sensor was prepared based on the zirconia thin porous film fabricated by cyclic voltammetric method in an aqueous electrolyte at room temperature.Because of the high sepecific surface of MWCNT,favorable electron transfer properties,biocompatibility of zirconia and high adsorption capacity of DNA,the immobilization of DNA probe and detection sensitivity of DNA hybridization were promoted.Using methylene blue（MB） as redox indicator,DNA immobilization and hybridization were characterized by cyclic voltammetry and differential pulse voltammetry.The sensor showed high sensitivity and good linearity,the peak current of MB was linearly related to the concentration of the target DNA in the range 2.15×10-7to 2.15×10-10 mol/L with the detection limit of 6.58×10-12 mol/L.