文献类型：期刊文献

英文题名：Study of lincomycin partition in a recyclable thermo-pH responsive aqueous two-phase system

作者：Wang, Yan[1];Yang, Ting[1];Zeng, Hainan[1];Wan, Junfen[1];Cao, Xuejun[1]

机构：[1]East China Univ Sci & Technol, Dept Bioengn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：109

起止页码：27

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20212710597672);WOS:【SCI-EXPANDED(收录号:WOS:000687771800004)】；

基金：The authors appreciate the financial support from National Natural Science Foundation of China (No. 21376078) .

语种：英文

外文关键词：Aqueous two-phase systems; Licomycin; pH-responsive polymer; Thermo-responsive polymer

摘要：Aqueous two-phase systems (ATPS) have been widely used in the laboratory separation and purification of antibiotics. However, its large-scale application is limited due to the difficulty in the recovery of the phase-forming polymers. In this study, we aimed to develop a thermo-pH responsive recyclable ATPS using thermoresponsive polymer EO30PO70 and pH-responsive polymer P-ADB5.22 for lincomycin partition. This ATPS can rapidly form two-phase interface in less than 5 mM and can sustainably be reused. The phase separation mechanism was studied by low-field nuclear magnetic resonance (LF-NMR) and surface tension. The effects of polymer concentration, lincomycin content, pH, temperature and salts type and concentrations on lincomycin partition were investigated. The 25 % (w/v) EO30PO70/4.5 % (w/v) P-ADB5.22 ATPS showed good partitioning at room temperature for lincomycin fermentation broth in the presence of 80 mM LiCl at pH 9.1, with a partition coefficient of 5.94 and extraction recovery of 94.68 %. In addition, the recycling of the two polymers showed a high efficiency, with 95.51 % and 96.18 % recoveries of EO30PO70 and P-ADB5.22 after four cycles, respectively. This recyclable ATPS has a promising industrial application in the separation and purification of antibiotics.