文献类型：期刊文献

英文题名：An association study between the genetic polymorphisms within GnRHI, LHβ and FSHβ genes and central precocious puberty in Chinese girls

作者：Zhao, Ying[1,2];Chen, Ting[1];Zhou, Yuxun[1];Li, Kai[1];Xiao, Junhua[1]

机构：[1]DongHua Univ, Inst Biol Sci & Technol, Shanghai 201620, Peoples R China;[2]E China Univ Sci & Technol, Coll Biochem & Mol Biol, Inst Bioengn, Shanghai 200237, Peoples R China

年份：2010

卷号：486

期号：3

起止页码：188

外文期刊名：NEUROSCIENCE LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000284565900016)】；

基金：This work was supported by grants from the National Nature Science Foundation of China (Grant No 30771191) and Science and Technology Commission of Shanghai Municipality (Grant No 071409004)

语种：英文

外文关键词：SNP; Linkage disequilibrium; CPP; LH beta; GnRHI; FSH beta

摘要：Objectives Gonadotropin-releasing hormone (GnRH) luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are three hypothalamic-pituitary-gonadal axis expressing hormones They play critical roles in the onset of puberty Here we report the relationship between the three hormones and Central Precocious Puberty (CPP) in Chinese Han girls Methods We analyzed the single nucleotide polymorphisms (SNPs) of 5'-flanking regions of these genes by DNA sequencing in 27 CPP samples Then the SNPs sites were genotyped by ligase detection reaction in a total of 283 Chinese Han CPP cases and 284 matched controls Distributions of the polymorphisms and haplotypes were calculated for statistical evaluation Results Nine SNPs (One in GnRHI gene -2003 C/T Five in LH beta gene -1456 C/G -1424 C/G -238 G/A -164 G/A and -34 T/A Three in FSH beta gene -1825 T/C -261 G/T and -132 T/A) were found A quantitative genetic association study was made -1825 T/C in FSH beta gene was related with CPP with a weak effect (P = 0 025) A haplotype in the 5'-flanking region of LH beta gene was significantly associated with CPP in Chinese Han girls (P = 8 25 x 10(-09)) However analysis software showed that none of SNP was found in the regulating control element of these genes Conclusions Our finding implies that the polymorphisms in the 5'-flanking regions of FSH beta gene and LH beta gene probably were related to the puberty onset time of these girls Further studies on the polymorphisms are needed for the exact mechanism (C) 2010 Elsevier Ireland Ltd All rights reserved