文献类型：期刊文献

英文题名：Quantitative determination of puerarin in dog plasma by HPLC and study on the relative bioavailability of sustained release tablets

作者：Ren, Fuzheng; Jing, Qiufang; Shen, Yongjia; Ma, Hongmei; Cui, Jingbin

机构：[1]E China Univ Sci & Technol, Dept Pharmaceut Engn, Sch Chem & Pharmaceut, Shanghai 200237, Peoples R China

年份：2006

卷号：41

期号：2

起止页码：549

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000237705700030)】；

语种：英文

外文关键词：puerarin; sustained release tablets; Yufengningxin tablets; bioavailability; HPLC

摘要：To evaluate the bioavailability of puerarin sustained release tablet (SR-Tab.) and Yufengningxin tablet (YU-Tab.), a liquid chromatography method was developed and validated to determine puerarin in dog plasma. Chromatographic separation was performed on Diamonsil C-18 column using a mixture of methanol-acetic acid-water (25:6:69, v/v/v) delivered at a flow rate of 1.0 ml/min and detected by UV. 4-Hydroxybenzaldehyde was used as the internal standard. The linear range for puerarin was from 60 to 1800 ng/ml (r = 0.9991) with a limit of quantitation of 60 ng/ml. Within-day accuracy and precision ranged from -3.0 to 2.2% and from 1.2 to 4.3%, between-day accuracy and precision ranged from -4.1 to 2.6% and from 1.3 to 5.7%, respectively. The mean extraction recoveries of puerarin determined over the three concentrations were (90.3 +/- 5.2)%, (95.7 +/- 1.4)% and (93.1 +/- 3.5)%. A significant difference was observed in main pharmacokinetic parameters of T-max C-max and AUC(0-infinity) between puerarin SR-Tab. and YU-Tab. in dogs. The smoother plasma concentrations were obtained from SR-Tab. in dogs and the results were as expected. (c) 2005 Elsevier B.V. All rights reserved.