文献类型：期刊文献

英文题名：In vitro toxicity of silica nanoparticles in myocardial cells

作者：Ye, Yiyi;Liu, Jianwen[1];Chen, Mingcang;Sun, Lijuan;Lan, Minbo

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：29

期号：2

起止页码：131

外文期刊名：ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000275539900006)】；

基金：The authors are grateful for financial support from the Nanotechnology special project of Shanghai (No. 0752nm025), Shanghai Leading Academic Discipline Project (Project no. B507) and the 111 Project (Grant no. B07023).

语种：英文

外文关键词：Silica nanoparticle; Oxidative stress; Cytotoxicity; Myocardial cell

摘要：In the present Study, toxicities of silica nanoparticles with sizes of 21 and 48 turn were assessed in myocardial H9c2(2-1) cells using the methylthiazolyldiphenyl-tetrazolium bromide reduction and lactate dehydrogenase assays. Cell injuries were characterized by morphological changes using hematoxylin and eosin staining. Reactive oxygen species, malondialdehyde and glutathione were measured to evaluate the levels of oxidative stress. To elucidate mechanisms, cell cycle distributions and the expressions of p53, p21 and Bax were also analyzed. Results showed that silica nanoparticles produced cytotoxicities in size, dose (0.1-1.6 mg/ml) and time (12, 24, 36 and 48 h exposure) dependent manners. Moreover, the particles caused oxidative stress, induced G1 phase arrest and upregulated levels of p53 and p21. Taken together, these data suggested that cell injuries were triggered by the generation of oxidative stress; p53 and p21 mediated G1 phase arrest is a potential mechanistic pathway of silica nanoparticles induced damage in H9c2(2-1) cells. (C) 2009 Elsevier B.V. All rights reserved.