文献类型：期刊文献

英文题名：Capabilities and challenges for the use of fluorescent RNAs in RNA dynamics research

作者：Fang, Mengyue[1,2];Jiang, Ying[1,2];Chen, Xianjun[1,2];Yang, Yi[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China

年份：2026

卷号：36

期号：2

起止页码：86

外文期刊名：TRENDS IN CELL BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001690233600002)】；

语种：英文

摘要：Visualizing RNA dynamics with high spatial and temporal resolution is key to un-Iderstanding their biological function. Fluorescent RNAs (FRs), which are fluores-cent protein (FP)-like entities comprising RNA aptamers and their cognate Fluorogenic dyes, provide an attractive approach for visualizing RNAs in live cells. In this opinion, we present the current potential of emerging FRs for real-time and multiplexed fluorescence imaging of RNA dynamics at the single mole-cule level or with super-resolution and provide guidance for the methodological and experimental factors that need to be considered when performing RNA im-aging by FRs, thus, increasing their effectiveness. We also provide perspectives and future directions for the development of FRs for advanced RNA imaging.