文献类型：期刊文献

英文题名：Brucea javanica oil-loaded nanostructure lipid carriers (BJO NLCs): Preparation, characterization and in vitro evaluation

作者：Lv, Wenhua[1,2];Zhao, Shuangni[1,2];Yu, Hui[4];Li, Na[5,6];Garamus, Vasil M.[7];Chen, Yiyin[1,2];Yin, Peihao[4];Zhang, Rongguang[5,6];Gong, Yabin[3];Zou, Aihua[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai 200437, Peoples R China;[4]Shanghai Univ Tradit Chinese Med, Putuo Hosp, Shanghai 200062, Peoples R China;[5]Natl Ctr Prot Sci Shanghai, Shanghai 200237, Peoples R China;[6]Shanghai Inst Biochem & Cell Biol, Shanghai 200237, Peoples R China;[7]Helmholtz Zentrum Geesthacht, Ctr Mat & Coastal Res, D-21502 Geesthacht, Germany

年份：2016

卷号：504

起止页码：312

外文期刊名：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

收录：;EI(收录号：20162402493701);WOS:【SCI-EXPANDED(收录号:WOS:000379836100035)】；

基金：We thank the staff at the BL19U2 beamline at the National Center for Protein Science Shanghai and the Shanghai Synchrotron Radiation Facility (Shanghai, People's Republic of China) for their assistance during data collection. We gratefully acknowledge the support of this work by Fundamental Research Funds for the Central Universities, the National Natural Science Foundation of China (Grant No. 21573070), the Natural Science Foundation of Shanghai (Grant No. 15ZR1409900) and the Knowledge Innovation Program of CAS (Grant No. 2013KIP103).

语种：英文

外文关键词：Brucea javanica oil; NLCs; SAXS; MTT; Cell flow cytometry

摘要：This study was aimed at preparing a stable nanostructure lipid carriers for Brucea javanica oil (BJO/NLCs) using the high-pressure homogenization (HPH) method. The obtained BJO/NLCs exhibited a particle size of approximately 180 nm and were quite stable during 30 days of storage. The entrapment efficiency (EE) and drug loading (DL) of BJO/NLCs were good, with values of up to 99.2% and 10.4%, respectively. The polydispersity index (PDI) and zeta potential values of BJO/NLCs were 0.17 and -31.2 mV, respectively. BJO/NLCs exhibited spherical or elongated particles when viewed by transmission electron microscopy (TEM). Small-angle X-ray scattering (SAXS) further confirmed that the internal structures of blank NLCs and BJO/NLCs were arranged into lamellar phases. Anti-tumor activity was studied using the MTT assay; the BJO/NLCs showed improved cytotoxicity to A549 cells. Taken together, these results indicate that this newly designed BJO/NLCs delivery system has the potential to exhibit improved BJO bioavailability. (C) 2016 Elsevier B.V. All rights reserved.