文献类型：期刊文献

英文题名：Tumour targeted contrast enhanced ultrasound imaging dual-modal microbubbles for diagnosis and treatment of triple negative breast cancer

作者：Bai, Min[1];Dong, Yang[2];Huang, Hui[2];Fu, Hao[2];Duan, Yourong[2];Wang, Qi[3,4];Du, Lianfang[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Gen Hosp, Dept Ultrasound, Shanghai 200080, Peoples R China;[2]Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Shanghai Canc Inst, Renji Hosp,Sch Med, Shanghai 200032, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2019

卷号：9

期号：10

起止页码：5682

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20190906565451);WOS:【SCI-EXPANDED(收录号:WOS:000459499900046)】；

基金：The authors acknowledge financial support from NSFC/China (81671687, 81771839 and 81602718), Fundamental Research Funds for the Central Universities (222201814013).

语种：英文

外文关键词：Diseases - Medical imaging - Shear stress - Cytology - Ultrasonic imaging - Image enhancement - Sulfur hexafluoride - Cell membranes - Tumors

摘要：At present, the treatment of triple negative breast cancer (TNBC) is a worldwide problem, urgently requiring early precise diagnosis and effective treatment methods. In our study, we designed a type of tumour targeted dual-modal microbubbles, paclitaxel (PTX)-loaded RGD-lipid microbubbles (PTX@ RGD-MBs), combined with ultrasonic targeted microbubble destruction (UTMD) to precisely diagnose TNBC and to improve the curative effect. As the first-line drug, PTX, lacking specific tumour targeting and the ability to be effectively internalized by TNBC cells, is still not effective in killing TNBC cells. For this reason, we used the tumour active targeting peptide RGD to precisely guide MBs around TNBC cells through the high affinity to the integrin avb3 receptor, and use UTMD to generate shear stress on cells to open transient pores in the cellular membrane, so as to improve MB penetration into the cellular membrane. Sulfur hexafluoride (SF6) as the internal gas of MBs can greatly improve the resolution and sensitivity of conventional US images through nonlinear harmonics to enhance the comparison between lesions and surrounding normal tissues. The results in our study demonstrated that RGD-MBs with UTMD were internalized by TNBC cells more effectively, leading to significant increase in intercellular drug concentrations of TNBC cells, thus achieving the best inhibitory effect on TNBC cells in vitro. Ultrasonic experiment showed that PTX@ RGD-MBs produced high quality contrast enhanced ultrasound (CEUS) images in vitro and in vivo, providing a better method for diagnosis and evaluation of the TNBC. Therefore, we conclude the advantages of excellent anti-tumour effect and CEUS imaging of PTX@ RGDMBs provided a better application for diagnosis and treatment of TNBC.