文献类型：期刊文献

中文题名：基因工程FR-008/杀念菌素脱羧衍生物CS103的分离提取及毒性和抗白色念珠菌活性研究

英文题名：Research on the Isolation,Cell Toxicity and Antifungal Activity Against Candida albicans of Novel Decarboxylated FR-008/Candicidin Derivative CS103

作者：毛相朝[1,2];陶欣艺[1];杨亮[1];沈亚领[1];魏东芝[1,2];邓子新[3]

机构：[1]华东理工大学生物反应器工程国家重点实验室鲁华生物技术研究所,上海200237;[2]中国科学院青岛生物能源与过程研究所中国科学院生物燃料重点实验室,山东青岛266101;[3]上海交通大学微生物代谢教育部重点实验室,上海200030

年份：2009

卷号：36

期号：12

起止页码：1859

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：上海市创新行动计划重大项目(No.2007DZ19503-4)

语种：中文

中文关键词：FR-008/杀念菌素,脱羧衍生物CS103,两性霉素B,毒性,抗真菌活性

外文关键词：FR-008/Candicidin, Novel decarboxylated derivative, Amphoteicin B, Toxicities, Antifungal activities

摘要：本文在建立了基因工程FR-008/杀念菌素脱羧衍生物CS103分离提取工艺的基础上,经进一步纯化,获得一定供试样品。通过对比脱羧FR-008/杀念菌素CS103、FR-008/杀念菌素和两性霉素B三种化合物对人胚肾细胞毒性、对人血红细胞的溶血活性和对白色念珠菌的抗菌效果,发现脱羧FR-008/杀念菌素CS103的毒性较FR-008/杀念菌素和两性霉素B大大降低,且保持了较高的抗真菌(Candida albicans)活性。
Based on founding the methods for isolation and purification of the novel decarboxylated FR-008/Candicidin derivative CS103, we obtained enough samples for testing from the culture mycelia of the mutant of Streptomyces FR-008. Through comparing the cell toxicities on Human Embryonic Kidney Cells 293, haemolytic activities on human erythrocytes and antifungal activities on C. albicans, we found that the toxicity of decarboxylated FR-008/Candicidin derivative CS103 had been lower than FR-008/Candicidin and Amphoteicin B, while it still had high antifungal activity on C. albicans.