文献类型：期刊文献

英文题名：A Reversible Dual-Channel Near-Infrared Flavonoid Probe for in Vivo Tracking Glutathione Dynamics in Living Mice

作者：Zhao, Zijun[1];Zhang, Yutao[1];Wu, Menglan[1];Yan, Chenxu[1];Guo, Zhiqian[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem,State K, Shanghai 200237, Peoples R China

年份：2023

卷号：1

期号：7

起止页码：620

外文期刊名：CHEMICAL & BIOMEDICAL IMAGING

收录：WOS:【ESCI(收录号:WOS:001356808900001)】；

基金：This work was supported by NSFC/China (22225805 and 32121005), China Postdoctoral Science Foundation (2021M701199), Shanghai Pujiang Program (Grant 21PJD038), and Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28).

语种：英文

外文关键词：Fluorescent probe; dual-channel; glutathione; reversible; bioimaging

摘要：Glutathione (GSH) plays a critical role in various biological processes maintaining oxidative homeostasis. However, current reversible probe fluorescence emission is usually in the visible region, making it difficult to monitor glutathione levels in deep tissues and in vivo. Here, we developed a reversible near-infrared fluorescence probe, Flav-N, for real-time tracking of GSH in cells and tissues, which undergoes fast and reversible Michael addition reactions with biothiols. This Flav-N probe showed a rapid and reversible response with GSH at a time of less than 5 s (k = 1286 M-1S-1, t 1/2 = 729 ms). Notably, the dynamic changes in the ratio of Flav-N emission intensity at 505 and 728 nm were able to provide real-time feedback on the fluctuation of GSH concentration. We demonstrated that Flav-N enables the performance of fast and reversible imaging of intracellular GSH changes. Importantly, in light of the near-infrared emission and rapid response ability, Flav-N was successfully applied to track GSH dynamics in living mice. This reversible near-infrared NIR probe realizes advances in deep insight into the function of endogenous GSH.