文献类型：期刊文献

英文题名：Immobilization of penicillin G acylase on paramagnetic polymer microspheres with epoxy groups

作者：Chen, Xing;Yang, Lu;Zhan, Wangcheng[1];Wang, Li;Guo, Yun;Wang, Yunsong;Lu, Guanzhong;Guo, Yanglong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Sch Chem & Mol Engn, Res Inst Ind Catalysis, Shanghai 200237, Peoples R China

年份：2018

卷号：39

期号：1

起止页码：47

外文期刊名：CHINESE JOURNAL OF CATALYSIS

收录：;EI(收录号：20180604757132);WOS:【SCI-EXPANDED(收录号:WOS:000428822100007)】；

基金：This work was supported by the National Natural Science Foundation of China (91545103), Shu Guang Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (10SG30), and the Fundamental Research Funds for the Central Universities (222201717003).

语种：英文

外文关键词：Paramagnetic polymer microspheres; Epoxy groups; Penicillin G acylase; Covalent bonding; Immobilization

摘要：Paramagnetic polymer microspheres were synthesized by the inverse suspension polymerization method through polymerization of glycidyl methacrylate, ally glycidyl ether and methacrylamide on the surface of silica-coated Fe3O4 nanoparticles using N,N'-methylene-bis(acrylamide) as a cross-linking agent Penicillin G acylase (PGA) was covalently immobilized on the surface of the paramagnetic microspheres by reacting the amino groups of the PGA molecules with the epoxy groups of the paramagnetic polymer microspheres. The effect of the SiO2 coating and the amount of paramagnetic Fe3O4 nanoparticles on the initial activity and the operational stability of the immobilized PGA was investigated. The results indicated that SiO2 played an important role in the polymerization process and paramagnetic polymer microspheres with a SiO2-coated Fe3O4 nanoparticles mass content of 7.5% are an optimal support material for PGA immobilization. Immobilized PGA on the paramagnetic polymer microspheres shows a high initial activity of 430 U/g (wet) and retains 99% of its initial activity after recycling 10 times. Furthermore, immobilized PGA exhibits high thermal stability, pH stability and excellent reusability, which can be rapidly recycled by the aid of magnet. (C) 2018, Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.