文献类型：期刊文献

英文题名：Quantitative analysis of free fatty acids in gout by disposable paper-array plate based MALDI MS

作者：Ren, Hongxin[1,2,3];Chen, Wanchao[1,2,3];Wang, Haiting[4];Kang, Yan[1,2,3];Zhu, Xixi[1,2,3];Li, Juan[1,2,3];Wu, Ting[1,2,3];Du, Yiping[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[4]Tech Ctr Ind Prod & Raw Mat Inspect & Testing, Shanghai Customs, Shanghai 200135, Peoples R China

年份：2019

卷号：579

起止页码：38

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000473557100006)】；

基金：This work was supported by Fundamental Research Funds for the Central Universities (No.222201714047), Science and Technology Commission of Shanghai Municipality (No. 17142202600) and Key Laboratory of Cigarette Smoke Research, CNTC (Shanghai Tobacco Group CO., LTD).

语种：英文

外文关键词：Disposable; Paper-array plate; MALDI TOF MS; Free fatty acids; Gout

摘要：The quantitative determination of small molecules by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF MS) was always challenging, due to low sensitivity and interferences of matrix and other contaminants on sample plates. Here we report a disposable paper-array (PA) plate in MALDI TOF MS analysis for quantitative analysis of serum free fatty acids (FFAs) with less interference and higher sensitivity, compared with conventional stainless steel (SS) plate. The disposable PA plate was low cost and environmentally friendly. In particular, it was more sensitive in FFAs detection with similar to 8-34-fold sensitivity increase for eight FFAs as compared to SS plate. Eight serum FFAs in gout patients were investigated using this disposable PA plate. The quantitative results showed good linearity of all eight FFAs between 0.1 and 2.5 mM with correlation coefficients between 0.994 and 0.999 and limits of detection (LODs) between 8.6 and 104.2 mu M. Mann-Whitney U test, principal component analysis (PCA) and random forest categorizer allowed the clear separation of gout patients from healthy controls, according to eight FFAs levels in serum detected by disposable PA plate based MALDI TOF MS.