文献类型：期刊文献

英文题名：A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A

作者：Hao, Liling[1];Wang, Wei[1];Shen, Xueqing[1];Wang, Shuliu[1];Li, Qian[3];An, Faliang[1];Wu, Shijia[2,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China;[3]Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Jiangsu, Peoples R China

年份：2020

卷号：68

期号：1

起止页码：369

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20200208021915);WOS:【SCI-EXPANDED(收录号:WOS:000507147100038)】；

基金：This work was partly supported by Natural Science Foundation of Shanghai (18ZR1409500), China Postdoctoral Science Foundation (2018M632047), the Fundamental Research Funds for the Central Universities (222201814029), National Natural Science Fund of China (NSFC 31772086), Jiangsu Agriculture Science and Technology Innovation Fund (CX(18)2025-01), and Young Elite Scientists Sponsorship Program by CAST (2017QNRC001).

语种：英文

外文关键词：DNA hydrogel; aptamer; rolling circle amplification; OTA

摘要：A sensitive fluorescent DNA hydrogel aptasensor based on the self-assembly of rolling circle amplification (RCA) products was developed for ochratoxin A (OTA) detection in beer. A competitive binding mode of aptamer, complementary sequence, and target was integrated into the DNA hydrogel for OTA detection. The OTA aptamer first combined with the primer to form the hybridized product. Then, in the presence of OTA, the aptamer combined with OTA, which released the primer. The released primer hybridized with the padlock probe to form a circular template, and the RCA reaction was initiated by adding ligase, polymerase, and dNTPs. The fluorescent DNA hydrogel was obtained by adding Cy3-dUTP together with dNTPs, and the fluorescence (FL) intensity of the DNA hydrogel was positively correlated with OTA concentration. Under the optimal experimental conditions, the linear range of the relationship varied from 0.05 ng/mL to 100 ng/mL with a detection limit for OTA of 0.01 ng/mL. The fluorescent DNA hydrogel aptasensor showed good specificity and stability in beer samples. Therefore, the fabricated DNA hydrogel aptasensor shows considerable potential applications in detecting OTA for food safety.