文献类型：期刊文献

英文题名：Co-production of 3-hydroxypropionic acid and 1,3-propanediol by Klebseilla pneumoniae expressing aldH under microaerobic conditions

作者：Huang, Yanna[1];Li, Zhimin[1];Shimizu, Kazuyuki[2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Kyushu Inst Technol, Grad Sch Comp Sci & Syst Engn, Dept Biosci & Bioinformat, Iizuka, Fukuoka 8208502, Japan

年份：2013

卷号：128

起止页码：505

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000315545800071)】；

基金：This study was supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204, and the National High Technology Research and Development Program of China, No. 2011AA02A203, 2012AA022104 and 2012AA021205.

语种：英文

外文关键词：3-Hydroxypropionic acid; 1,3-Propanediol; Klebsiella pneumoniae; Aldehyde dehydrogenase; Aeration

摘要：Fed-batch cultures of Klebsiella pneumoniae expressing Escherichia coli aldH were performed under microaerobic conditions to investigate the effects on metabolites production. Increasing the aeration rate enhanced cell growth and 3-hydroxypropionic acid (3-HP) production, but reduced 1,3-propanediol (1,3-PDO) formation. The recombinant strain K. pneumoniae/pUC18kan-aldHec produced 48.9 g/L of 3-HP and 25.3 g/L of 1,3-PDO with an overall yield of 0.66 mol/mol in 28 h at an aeration rate of 1.5 vvm; however, under fully aerobic condition, no 3-HP and 1,3-PDO were produced due to the repression of dha operon. The flux through the reaction catalyzed by glycerol dehydratase and the split ratio of 1,3-PDO were negatively correlated with the aeration rate, even though the 3-HP level showed a positive trend. This study demonstrated that the relative amounts of 3-HP and 1,3-PDO can be controlled by the aeration rate. (C) 2012 Elsevier Ltd. All rights reserved.