文献类型：期刊文献

英文题名：Inhibition of tumor cell proliferation by coleon C

作者：Xing, Xiu[1,2];Wu, Hezhen[3];Wang, Xiaoming[3];Huang, Yongping[1,2];Li, Qing[1,2];Li, Changlong[1,2];Yang, Yanfang[3];Liu, Yanwen[3];Liu, Jianwen[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[3]Hubei Coll Tradit Chinese Med, Key Lab Resources & Chem Chinese Med, Wuhan 430061, Hubei, Peoples R China

年份：2008

卷号：20

期号：2

起止页码：238

外文期刊名：JOURNAL OF CHEMOTHERAPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000256452800015)】；

语种：英文

外文关键词：tumor; coleon C; DNA fragmentation; apoptosis; Lewis lung carcinoma

摘要：Coleon C (6,11,12,14,16-pentahydroxyabieta-5,8,11,13-tetraen-7-one), extracted from Coleus forskohlii Briq., was investigated for its anti-tumor activity on eight human tumor cell lines (95-D, A375, HeLa, A431, MKN45, BEL7402, LoVo and HI-60) and two normal ones (293, L02) by MTT and colony-forming assay in vitro. The results indicated that A375 was the most sensitive of all the cell lines. Hoechst 33258 staining showed fragmentation and condensation of chromatin. DNA ladder assay indicated the fragments of DNA because of apoptosis. Flow cytometric analysis demonstrated hypodiploid cells existed in A375 after Coleon C treatment. In the acute toxicity studies of C57BL/6 mice, LD50 of Coleon C was 1496 +/- 150 mg/kg. In the model of Lewis lung carcinoma, the average tumor weight in groups injected with 80 mg/kg Coleon C decreased by 48.9 +/- 14.3% compared with that of the control. These results indicate that Coleon C could effectively inhibit tumor cell proliferation and growth by inducing apoptosis with low toxicity. To our knowledge, this is the first report on the anti-tumor activity of Coleon C both in vitro and in vivo.