文献类型：期刊文献

英文题名：A Photo-triggered and photo-calibrated nitric oxide donor: Rational design, spectral characterizations, and biological applications

作者：He, Haihong[1];Liu, Yuxin[7];Zhou, Zhongneng[3];Guo, Chunlei[1];Wang, Hong-Yin[5];Wang, Zhuang[1];Wang, Xueli[3];Zhang, Ziqian[6];Wu, Fu-Gen[5];Wang, Haolu[2];Chen, Daijie[4];Yang, Dahai[1];Liang, Xiaowen[2];Chen, Jinquan[3];Zhou, Shengmin[1];Liang, Xin[1];Qian, Xuhong[1];Yang, Youjun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design,Sch Pharm, State Key Lab Bioreactor Engn,Shanghai Key Lab Ch, Shanghai 200237, Peoples R China;[2]Univ Queensland, Translat Res Inst, Diamantina Inst, Therapeut Res Ctr, Woolloongabba, Qld 4102, Australia;[3]East China Normal Univ, State Key Lab Precis Spect, Shanghai 200062, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China;[5]Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China;[6]Guangxi Univ Chinese Med, Guangxi Sci Res Ctr Tradit Chinese Med, Nanning 530200, Guangxi, Peoples R China;[7]Nanjing Univ Sci & Technol, Sch Environm & Biol Engn, Nanjing 210094, Jiangsu, Peoples R China

年份：2018

卷号：123

起止页码：1

外文期刊名：FREE RADICAL BIOLOGY AND MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000436459200001)】；

基金：The work is supported by the Fundamental Research Funds for the Central Universities (Nos. WY1514053, WY1516017), Shanghai Pujiang Program (16PJ1402500) and the National Natural Science Foundation of China (Nos. 21372080, 21572061, 11674101, 21236002, and 81502540).

语种：英文

外文关键词：Nitrc oxide donors; Photo-triggered; Photo-calibrated; Mesenchymal stem cell migration

摘要：Nitric oxide (NO) donors are valuable tools to probe the profound implications of NO in health and disease. The elusive nature of NO bio-relevance has largely limited the use of spontaneous NO donors and promoted the development of next generation NO donors, whose NO release is not only stimulated by a trigger, but also readily monitored via a judiciously built-in self-calibration mechanism. Light is without a doubt the most sensitive, versatile and biocompatible method of choice for both triggering and monitoring, for applications in complex biological matrices. Herein, we designed and synthesized an N-nitroso rhodamine derivative (NOD560) as a photo-triggered and photo-calibrated NO donor to address this need. NOD560 is essentially non-fluorescent. Upon irradiation by green light (532 nm), it efficiently release NO and a rhodamine dye, the dramatic fluorescence turn-on from which could be harnessed to conveniently monitor the localization, flux, and dose of NO release. The potentials of NOD560 for in vitro biological applications were also exemplified in in vitro biological models, i.e. mesenchymal stem cell (MSC) migration suppression. NOD560 is expected to complement the existing NO donors and find widespread applications in chemical biological studies.