文献类型：期刊文献

英文题名：A low-cost multi-layer microfluidic platform for on-site viability assessment of stem cells at single-cell resolution☆

作者：Xiao, Wenjie;Guo, Jiahao;Zhao, Xusheng;Huang, Jiacheng;Jiang, Guojun[1];Ouyang, Liming[1];Gu, Zhen[1];Zhang, Lixin;Wang, Huifeng

机构：[1]East China Univ Sci & Technol, Minist Educ, Key Lab Smart Mfg Energy Chem Proc, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：214

外文期刊名：MICROCHEMICAL JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001496949500003)】；

基金：This research was funded by National Major Scientific Instruments and Equipments Development Project of National Natural Science Foundation of China (grant number: 32327801) , the National Natural Science Foundation of China (grant number: 62103148) and the Shanghai Science and Technology Commission (contract number: 23J21900300) .

语种：英文

外文关键词：Microfluidic chip; Electrical impedance signal; Cell-specific adhesion; Label-free detection; On-site viability assay; Single-cell resolution

摘要：The detection of stem cell activity is crucial for regenerative medicine and cell therapy. However, conventional methods for assessing cell viability are often time-consuming, costly, and result in the non-reusability of cells. In response to these challenges, we developed a low cost and label-free method for detection of cell viability with single-cell resolution, which is implemented by an integrated multi-layer microfluidic system (MLMS) enabling both cell culture and impedance sensing in real time. MLMS integrates a live cell-specific adhesion mechanism with single-cell microfluidic impedance detection. By the selective capture of living cells by fibronectin, live and dead cells can be counted separately, thereby allowing for accurate viability assessment. Fluorescent polystyrene microbeads were counted using both image recognition and impedance detection, demonstrating the high counting accuracy of MLMS. The detection accuracy of chips with micropores of various diameters was assessed using polystyrene (PS) beads of varying sizes. The results show that the chip with 60 mu m micropores exhibits superior resolution and enables comprehensive cell detection. Furthermore, mixtures containing different ratios of live and dead cells were analyzed to assess cell viability using the MLMS. The obtained results were very close to the expected values. This work introduces a innovative low-cost, label-free, single-cell resolution solution for cell viability detection. Thereby, it hold promising application prospects in the monitoring and control of cell morphology and quantity during the stem cell culture process.