文献类型：期刊文献

英文题名：Efficient strategies for preparative separation of iridoid glycosides and flavonoid glycosides from Hedyotis diffusa

作者：Dai, Yingping[1];Zhang, Hongzhi[1];Wang, Xinhe[1];Chen, Yanchun[1];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

年份：2023

卷号：46

期号：10

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20231313802319);WOS:【SCI-EXPANDED(收录号:WOS:000950298300001)】；

语种：英文

外文关键词：flavonoid glycosides; Hedyotis diffusa; iridoid glycosides; preparative high-performance liquid chromatography

摘要：Efficient strategies for the preparative separation of iridoid glycosides and flavonoid glycosides from Hedyotis diffusa using preparative high-performance liquid chromatography combined with appropriate pretreatment technologies were developed. Four fractions (Fr.1-1, Fr.1-2, Fr.1-3, and Fr.2-1) were firstly isolated from the crude extract of Hedyotis diffusa by column chromatography with C18, resin, and silica gel materials, respectively. Then, corresponding separation strategies were developed according to the polarity and chemical constituents. High-polar compounds of Fr.1-1 were purified by hydrophilic reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography mode. The combination of C18 and phenyl columns realized the complementary separation of iridoid glycosides in Fr.1-2. Meanwhile, the improved selectivity caused by the change of organic solvent in the mobile phase was utilized to realize the purification of flavonoid glycosides in Fr.1-3 and Fr. 2-1. Finally, 27 compounds (purity > 95%) mainly involving nine iridoid glycosides and five flavonoid glycosides were obtained. A complete strategy was established for the separation of a complex sample with a wide polarity range, to jointly solve the problems of enrichment of target components and separation of structural analogs.