文献类型：期刊文献

中文题名：杂交瘤细胞的无血清低蛋白培养

英文题名：Adaptation of Hybridoma Cells to Serum-free Low-protein Culture

作者：张淑香[1];李东晓[1];朱明龙[1];谭文松[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：29

期号：4

起止页码：372

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2003447708005);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：上海市启明星计划(98QF1403);上海市高等学校青年科学基金

语种：中文

中文关键词：杂交瘤细胞;无血清低蛋白;适应;比生长速率;代谢

外文关键词：hybridoma cells; serum-free low-protein; adaptation; specific growth rate; metabolism

摘要：实现了杂交瘤细胞的体外无血清低蛋白培养。考察了血清浓度、基础培养基和接种密度对细胞生长的影响。在含5%血清培养基、1%血清培养基和无血清低蛋白培养基中适应生长的细胞,μ分别为0.68、0.45、0.44d-1,qGlc分别为13.4×10-9、13.1×10-9、19.2×10-9mmol/(cell·分别为39.4×10-9、44.1×10-9、37.7×10-9mg/(cell·d),YMAb/Xv分别为52.6×10-9、d),qMAb57.8×10-9、54.4×10-9mg/cell,YLac/Glc分别为2.0、1.95、2.0mmol/mmol。无血清培养中葡萄糖代谢途径不变,参与细胞维持代谢的葡萄糖比例增大。血清浓度降低,延迟期变长,最大活细胞密度下降。基础培养基影响细胞的最大活细胞密度,不影响生长速率。无血清培养基中细胞的接种密度选择在0.2×106～0.3×106cells/mL。
Hybridoma cells were successfully cultured in vitro in serumfree lowprotein medium. The effects of serum、basal medium and inoculum on cell growth were investigated and sfm was optimized. The specific growth rates of cells normally growing in the media containing 5%NBS、1%NBS and serumfree lowprotein media were 0.68、0.45、0.44 d-1, specific consumption rates of glucose 13.4×10-9、13.1×10-9、19.2×10-9 mmol/(cell·d), specific production rates of MAb were 59.4×10-9、44.1×10-9、37.7×10-9 mg/(cell·d), yields of MAb from viable cell density were 52.6×10-9、57.8×10-9、54.4×10-9 mg/cell, yields of lactate from glucose were 2.0、1.95、2.0 mmol/mmol respectively. Metabolic pathways did not change, but more glucose was consumed for maintenance metabolism in serumfree culture. With low serum concentration, lag phase in batch mode was prolonged and maximum viable cell densities reduced. The basal media affected maximum viable cell density, not specific growth rate. The suitable inocula of cells cultured in serumfree medium lay between 0.2×106～0.3×106 cells/mL.