文献类型：期刊文献

英文题名：Chitobiose alleviates oleic acid-induced lipid accumulation by decreasing fatty acid uptake and triglyceride synthesis in HepG2 cells

作者：Li, Xiaodan[1];Zhao, Mengyao[1];Fan, Liqiang[1];Cao, Xuni[1];Chen, Liehuan[3];Chen, Junhui[4];Lo, Y. Martin[2];Zhao, Liming[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Fuzhou Univ, Fuzhou 350116, Fujian, Peoples R China;[3]Huizhou Long Dragon Biotechnol Co Ltd, Huizhou 516000, Peoples R China;[4]Peking Univ, Shenzhen Hosp, Dept Minimally Invas Intervent, Shenzhen 518036, Peoples R China

年份：2018

卷号：46

起止页码：202

外文期刊名：JOURNAL OF FUNCTIONAL FOODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000443630000022)】；

基金：This work is financially supported by the National Natural Science Foundation of China (No. 31371725), The National Key Research and Development Program of China (2017YFB0309302) and 'Shu Guang' project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (15SG28).

语种：英文

外文关键词：Chitobiose; Hepatic steatosis; Oleic acid; CD36; DGAT2

摘要：This study investigates the ameliorative effect of crude chitooligosaccharide (COS) and five specific COSs ((GlcN)(2-6)) on lipid accumulation and, therein, characterizes the inhibition mechanism of chitobiose ((GlcN)(2)). After treatment with oleic acid (OA), the triglyceride (TG), LDL-c content, lipogenesis-signaling genes and protein in HepG2 cells increased, while lipid accumulation was suppressed by COS and five single COSs both in co-treatment and after-treatment. In addition, we observed that 4 mg/mL (GlcN)(2) had a significant inhibitory effect on hepatic lipid accumulation and decreased the mRNA and protein expressions of diacylglycerol acyltransferase 2 (DGAT2), liver X receptor alpha (LXR alpha), peroxisome proliferator-activated receptor-gamma (PPAR gamma), pregnenolone X receptor (PXR) and cluster of differentiation 36 (CD36). These results collectively indicate that, among all (GlcN)(2-6) studied, (GlcN)(2) provides the best active effect on anti-hyperlipidemia and steatosis regulation via decreasing fatty acid uptake and TG synthesis in HepG2 cells.