文献类型：期刊文献

英文题名：Spiropyran in Situ Switching: A Real-Time Fluorescence Strategy for Tracking DNA G-Quadruplexes in Live Cells

作者：Li, Jin[1];Yin, Xinchi[2];Li, Bin[1];Li, Xiaokang[3];Pan, Yuanjiang[2];Li, Jian[3];Guo, Yuan[1]

机构：[1]Northwest Univ, Key Lab Synthet & Nat Funct Mol Chem, Key Lab Resource Biol & Biotechnol Western China, Minist Educ,Coll Chem & Mat Sci,Natl Demonstrat C, Xian 710127, Shaanxi, Peoples R China;[2]Zhejiang Univ, Dept Chem, Hangzhou 310058, Zhejiang, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：91

期号：8

起止页码：5354

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20191606775251);WOS:【SCI-EXPANDED(收录号:WOS:000465189600058)】；

基金：The authors acknowledge the National Natural Science Foundation of China (No. 21472148, 21072158, and 21532005) and Open Funding Project of the State Key Laboratory of Bioreactor Engineering and Academic Backbone of Northwest University Outstanding Youth Support Program. The authors thank Guangfu Yang at Central China Narmal University, Minyong Li at Shandong University, and Sanping Chen and Xi Chen at Northwest University for insightful discussions on this work. The authors thank Xiaoting Li and Peng Ji at the Demo Laboratory of ZEISS Microscopy Customer Centers in Shanghai for assistance with super resolution imaging. The authors also thank Qingfeng Xiao and Zhifeng Cheng at Nikon Biological Imaging Center in Shanghai for assistance with SIM imaging.

语种：英文

外文关键词：Cytology - Fluorescence imaging - Cells - Diagnosis - Red Shift

摘要：DNA G-quadruplexes (G4s) in vivo have been linked to cancer and other diseases such as neurological disorders. Non-destructive fast detection of endogenous DNA G4s can provide specific real-time information, which is of particular interest for clinic accurate diagnosis. However, tools to probe live-cell endogenous DNA G4s in real time are very limited. Herein, we report the design and development of a fluorescent molecule QIN for the real-time detection of endogenous DNA G4s in live cells with the aid of a new spiropyran in situ switching (SIS) strategy. The lipophilic spiropyran-linked QIN differs from the other probes in that it can enter live cells readily within 15 s and can be in situ induced by DNA G4s to adopt its charged open form, causing a large red shift in the fluorescent emission wavelength. Live-cell super-resolution fluorescent imaging suggests that the SIS-based probe has high photostability and can be applied for the accurate detection of DNA G4s in complex biosystems with very high sensitivity and selectivity.