文献类型：期刊文献

英文题名：Super-Resolution Monitoring of Mitochondrial Dynamics upon Time-Gated Photo-Triggered Release of Nitric Oxide

作者：He, Haihong[1];Ye, Zhiwei[2];Xiao, Yi[2];Yang, Wei[2];Qian, Xuhong[1];Yang, Youjun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Liaoning, Peoples R China

年份：2018

卷号：90

期号：3

起止页码：2164

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20180604777520);WOS:【SCI-EXPANDED(收录号:WOS:000424730600095)】；

基金：The work is financially supported by the National Natural Science Foundation of China (Nos. 21372080, 21236002, 21376038, 21421005, 21572061, 21576040, and 21776037), National Basic Research Program of China (No. 2013CB733702), and the Fundamental Research Funds for the Central Universities (Nos. WY1514053, WY1516017, and DUT17LK43).

语种：英文

外文关键词：Dynamics - Mitochondria

摘要：Nitric oxide (NO) potentially plays a regulatory role in mitochondrial fusion and fission, which are vital to cell survival and implicated in health, disease, and aging. Molecular tools facilitating the study of the relationship between NO and mitochondrial dynamics are in need. We have recently developed a novel NO donor (NOD550). Upon photoactivation, NOD550 decomposes to release two NO molecules and a fluorophore. The NO release could be spatially mapped with subdiffraction resolution and with a temporal resolution of 10 s. Due to the preferential localization of NOD550 at mitochondria, morphology and dynamics of mitochondria could be monitored upon NO release from NOD550.