文献类型：期刊文献

英文题名：Design and Synthesis of New Acid Cleavable Linkers for DNA Sequencing by Synthesis

作者：Jiang, Min[1,2];Tang, Daonian[4,5];Zhao, Xiaodong[3];Li, Qing[4,5];Zhuang, Yuan[4,5];Wei, Xiaofei[1,2];Li, Xiaowei[3];Liu, Yazhi[4,5];Wu, Xin-Yan[4,5];Shao, Zhifeng[1,2,3];Gong, Bing[6,7];Shen, Yu-Mei[1,2]

机构：[1]Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Shanghai 200240, Peoples R China;[2]Shanghai Jiao Tong Univ, BioID Ctr, Shanghai 200240, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China;[4]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[6]Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China;[7]SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA

年份：2014

卷号：33

期号：12

起止页码：774

外文期刊名：NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344399600003)】；

基金：We acknowledge the financial support provided by the National Natural Science Foundation of China (81071250 and 91227109), Major Projects in National Science and Technology, "Creation of Major New Drugs" (2011ZX09501-001-05), and the US National Science Foundation (CHE-1306326).

语种：英文

外文关键词：synthetic methodology; fluorescent base analogues; Triphosphate analogues

摘要：A new kind of acid sensitive tetrahydrofuranyl (THF) linker was synthesized and then reacted with 5-(6)-carboxytetramethylrhodaminesuccinimidyl ester (5(6)-TAMRA, SE), followed by di(N-succinimidyl) carbonate (DSC) and modified 2 '-deoxyuridine triphosphate (dUTP); the final product, as a reversible terminator for DNA sequencing by synthesis (DNA SBS), was given obtained and confirmed by H-1-NMR, P-31-NMR, and HRMS with purity of up to 99%. The synthesized dye-labeled terminator incorporated into DNA strand successfully, and the fluorophore was cleaved completely under acidic conditions. The preliminary results encourage us to explore more acid-sensitive linkers for DNA SBS to increase the cleavage efficiency under weakly acidic conditions.