文献类型：期刊文献

英文题名：Sandwich-type electrochemical aptasensor based on coral-like gold-modified electrode and porous PtPdCu label for the sensitive detection of cardiac troponin I

作者：Chen, Kaicha[1,2,3];Zhao, Hongli[1];Wang, Zhenxing[1];Yu, Yueying[1];Lan, Minbo[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Yunnan Precious Met Lab Co Ltd, Kunming 650106, Peoples R China;[3]Sino Platinum Elect Mat Yunnan Co LTD, Kunming 650106, Peoples R China

年份：2025

卷号：43

外文期刊名：MATERIALS TODAY CHEMISTRY

收录：;EI(收录号：20250117635815);WOS:【SCI-EXPANDED(收录号:WOS:001398253400001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, 21JM0010400) , and the Funda-mental Research Funds for the Central Universities, China (JKJ01241717) .

语种：英文

外文关键词：Sandwich-type; Cardiac troponin I; Platinum-palladium-copper; Electrochemical aptasensor

摘要：This work presented a sandwich-type electrochemical aptasensor for the sensitive determination of cardiac troponin I (cTnI). This aptasensor was constructed based on coral-like gold-modified electrode (CGE) as the substrate electrode and porous platinum-palladium-copper nanosphere (p-PtPdCu) as the label. The CGE was obtained by using a constant-current electrodeposition method. Due to its special coral-like structure, the conductivity of CGE was better than those of other gold-based electrodes. Moreover, the large effective area of the CGE was beneficial for fixing more aptamer (Tro4), which enhanced the electrochemical properties of the aptasensor. Meanwhile, the label p-PtPdCu was synthesized by a one-pot heating process. The label showed good electrocatalytic ability and can effectively amplify electrocatalytic signals because of its porous structure and synergistic effect, which was good for the sensing process. The detection of captured cTnI was accomplished by adding hydrogen peroxide (H2O2) into the buffer and recording the current response of p-PtPdCu towards H2O2. The magnitude of the current signal indirectly revealed the concentration of cTnI. As a result, the prepared aptasensor displayed excellent performance when detected cTnI, including wide linear range (100 ng/mL-10 fg/ mL), high sensitivity, low detection limit (1.68 fg/mL, S/N = 3), good recovery in human serum. These results proved the significance of the prepared CGE and p-PtPdCu, and the promising usage of this aptasensor in the diagnosis of cTnI.