文献类型：期刊文献

英文题名：Surface morphology and action mechanism of macrophage immunostimulating activity of Rosa Setate x Rosa Rugosa polysaccharide WSRP-1b

作者：Wu, Mengqi[1,2];Xu, Jingying[2];Li, Wei[3];Han, Qifeng[2];Xia, Wei[2];Li, Zhong[1];Zhang, Yang[1];Zhang, Wenqing[2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]Bright Dairy & Food Co Ltd, Dairy Res Inst, State Key Lab Dairy Biotechnol, Shanghai Engn Res Ctr Dairy Biotechnol, Shanghai 200436, Peoples R China

年份：2023

卷号：6

外文期刊名：CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS

收录：WOS:【ESCI(收录号:WOS:001041052100001)】；

基金：This work was co -financed by "Science and Technology Innovation Action Plan" for Shanghai Municipal Science and Technology Support Project (Grant No. 22S21902800) and the Scientific Fund of Shanghai Engineering Center of Dairy Biotechnology (Grant No. 19DZ2281400) .

语种：英文

外文关键词：kRosa Setate x Rosa Rugosa waste; Polysaccharide; Morphological features; Macrophages; Immunomodulation mechanism

摘要：Our previous research demonstrated that the glucomannan WSRP-1b, which was obtained from waste biomass of Rosa Setate x Rosa Rugosa, had potent immunostimulatory activity. However, the specific mechanisms involved in the activation of RAW264.7 macrophages by WSRP-1b remain unclear. Scanning electron microscopy, atomic force microscopy and Congo red analyses revealed that WSRP-1b exhibited a flake structure with irregular curls and had a triple-helix conformation in solution. The immunostimulation assays showed that WSRP-1b could substantially enhance macrophages phagocytosis and promote the accumulation of iNOS, TNF-& alpha;, and IL-6 mRNA. Western blot analysis indicated that the expression of mitogen-activated protein kinases (MAPKs) was upregulated in WSRP-1b-induced RAW264.7 cells. Furthermore, pretreatment of RAW 264.7 cells using specific inhibitors against MAPKs and NF-& kappa;B remarkably attenuated nitric oxide production and cytokine secretion. These data suggest that WSRP-1b activates RAW264.7 macrophages via the MAPK and NF-& kappa;B signaling pathways and may be a novel immunomodulatory agent for hypoimmunity disease therapy.