文献类型：期刊文献

英文题名：Doped Graphene To Mimic the Bacterial NADH Oxidase for One-Step NAD+ Supplementation in Mammals

作者：Liu, Xi[1]; Li, Jingkun[2]; Zitolo, Andrea[3]; Gao, Meng[1]; Jiang, Jun[1]; Geng, Xiangtian[4]; Xie, Qianqian[1]; Wu, Di[1]; Zheng, Huizhen[1]; Cai, Xiaoming[4]; Lu, Jianmei[5]; Jaouen, Frédéric[6]; Li, Ruibin[1]

机构：[1] State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences [RAD-X], Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou Medical College, Soochow University, Suzhou, 215123, China; [2] School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China; [3] L’orme des Merisiers, Synchrotron SOLEIL, BP 48 Saint Aubin, Gif-sur-Yvette, 91192, France; [4] School of Public Health, Suzhou Medical College, Soochow University, Suzhou, 215123, China; [5] College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123, China; [6] ICGM, CNRS, ENSCM, Univ. Montpellier, Montpellier, 34293, France

年份：2023

卷号：145

期号：5

起止页码：3108

外文期刊名：Journal of the American Chemical Society

收录：EI(收录号：20230713588947)

语种：英文

外文关键词：Bacteria - Catalyst activity - Cell culture - Doping (additives) - Graphene - Mammals - Metabolism - Transition metals

摘要：Nicotinamide adenine dinucleotide (NAD) is a critical regulator of metabolic networks, and declining levels of its oxidized form, NAD+, are closely associated with numerous diseases. While supplementing cells with precursors needed for NAD+ synthesis has shown poor efficacy in combatting NAD+ decline, an alternative strategy is the development of synthetic materials that catalyze the oxidation of NADH into NAD+, thereby taking over the natural role of the NADH oxidase (NOX) present in bacteria. Herein, we discovered that metal-nitrogen-doped graphene (MNGR) materials can catalyze the oxidation of NADH into NAD+. Among MNGR materials with different transition metals, Fe-, Co-, and Cu-NGR displayed strong catalytic activity combined with >80% conversion of NADH into NAD+, similar specificity to NOX for abstracting hydrogen from the pyridine ring of nicotinamide, and higher selectivity than 51 other nanomaterials. The NOX-like activity of FeNGR functioned well in diverse cell lines. As a proof of concept of the in vivo application, we showed that FeNGR could specifically target the liver and remedy the metabolic flux anomaly in obesity mice with NAD+-deficient cells. Overall, our study provides a distinct insight for exploration of drug candidates by design of synthetic materials to mimic the functions of unique enzymes (e.g., NOX) in bacteria. ? 2023 American Chemical Society.