文献类型：会议论文

英文题名：Screening of Oleaginous Yeast Strains Tolerant to Lignocellulose Degradation Compounds

作者：Chen, Xi[1];Li, Zihui[1];Zhang, Xiaoxi;Hu, Fengxian[1];Ryu, Dewey D. Y.[2];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Calif Davis, Biochem Engn Program, Davis, CA 95616 USA

会议论文集：12th Asia-Pacific-Confederation-of-Chemical-Engineering (APCChE 2008)

会议日期：AUG 04-06, 2008

会议地点：Dalian, PEOPLES R CHINA

语种：英文

外文关键词：Triacylglycerol lipid; Oleaginous yeasts; Trichosporon cutaneum; Screening; Lignocellulose degradation compounds

摘要：High cost of triacylglycerol lipid feedstock is the major barrier for commercial production of biodiesel. The fermentation of oleaginous yeasts for lipid production using lignocellulose biomass provides a practical option with high economic competitiveness. In this paper, the typical oleaginous yeast strains were screened under the pressure of lignocellulose degradation compounds for selection of the optimal strains tolerant to lignocellulose. The inhibitory effect of lignocellulose degradation products on the oleaginous yeast fermentation was carefully investigated. Preliminary screening was carried out in the minimum nutritious medium without adding any expensive complex ingredients then was carried out in the lignocellulosic hydrolysate pretreated by dilute sulfuric acid. Seven typical lignocellulose degradation products formed in various pretreatment and hydrolysis processing were selected as the model inhibitors, including three organic acids, two furan compounds, and two phenol derivatives. The inhibition of the degradation compounds on the cell growth and lipid productivity of the selected oleaginous yeasts were examined. Acetic acid, formic acid, furfural, and vanillin were found to be the strong inhibitors for the fermentation of oleaginous yeasts, while levulinic acid, 5-hydroxymethylfurfural, and hydroxybenzaldehyde were relatively weak inhibitors. Trichosporon cutaneum 2.1374 was found to be the most adopted strain to the lignocellulose degradation compounds.