文献类型：期刊文献

英文题名：Involvement of LuxS in the regulation of motility and flagella biogenesis in Vibrio alginolyticus

作者：Tian, Yang[1];Wang, Qiyao[1];Liu, Qin[1];Ma, Yue[1];Cao, Xiaodan[1];Guan, Lingyu[1];Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：72

期号：4

起止页码：1063

外文期刊名：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000255501100019)】；

语种：英文

外文关键词：flagella; LuxS; motility; quorum sensing; Vibrio alginolyticus

摘要：The fish pathogen Vibrio alginolyticus contains two unique flagellar systems. The LuxS quorum sensing system is reported to regulate the expression of virulence factors in a wide variety of pathogenic bacteria. Our previous work demonstrated that inactive luxS led to decreased virulence in V. alginolyticus. In this study, LuxS-dependent regulation of motility and flagella biogenesis, the potential virulence factors in V. alginolyticus, were further investigated. A luxS-deleted mutant showed deficiency in motility and flagella formation, and an intact luxS complemented the defect. Since motility is flagella dependent, V. alginolyticus flagella biogenesis related genes, including the flagella regulator genes flaK and lafK and the sub-hierarchical flagellar genes fliS and lafA, were cloned and identified. Based on quantitative real-time reverse transcription-PCR, differential expression of these genes was confirmed in wild-type and luxS mutants. Our results indicate that LuxS plays an important role in the regulation of motility and flagella biogenesis in V. alginolyticus.