文献类型：期刊文献

英文题名：Target-Triggered NIR Emission with a Large Stokes Shift for the Detection and Imaging of Cysteine in Living Cells

作者：Zhao, Chunchang[1,2];Li, Xiuai[1,2];Wang, Feiyi[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：9

期号：7

起止页码：1777

外文期刊名：CHEMISTRY-AN ASIAN JOURNAL

收录：;EI(收录号：20142717881601);WOS:【SCI-EXPANDED(收录号:WOS:000338017800007)】；

基金：We gratefully acknowledge the financial support by the National Science Foundation of China (grant no.: 21172071, 21190033, 21372083), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：biosensors; biothiols; excited state intramolecular proton transfer; fluorescent probes; imaging agents; NIR emission

摘要：Background autofluorescence from biological systems generally reduces the sensitivity of a fluorescent probe for imaging biological targets. Addressing this challenge requires the development of fluorescent probes that produce emission in the near-infrared region. Herein, we report the design and synthesis of a fluorescent probe that generates an NIR emission with a large Stokes shift upon the selective response to Cys over Hcy and GSH. The probe is designed to consist of two Cys-sensing sites, an acrylate ester and an aldehyde installed ortho to each other. The reaction of the probe with Cys triggers an excited state intramolecular proton transfer process upon photo-excitation, thereby producing an NIR emission with a large Stokes shift. Accordingly, this probe hold great promise for the selective detection of Cys in biological systems. We further demonstrate the capacity of this probe for Cys imaging in living cells.