文献类型：期刊文献

英文题名：Curcumin-based polarity fluorescent probes: Design strategy and biological applications

作者：Hu, Linghao[1];Shi, Donglei[1,2];Li, Xinming[1];Zhu, Jin[1];Mao, Fei[1];Li, Xiaokang[1];Xia, Conglong[3];Jiang, Bei[3];Guo, Yuan[2];Li, Jian[1,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Northwest Univ, Coll Chem & Mat Sci, Key Lab Synthet & Nat Funct Mol Chem, Minist Educ, Xian 710127, Peoples R China;[3]Dali Univ, Coll Pharm & Chem, Inst Mat Med, 5 Xue Ren Rd, Dali 671000, Yunnan, Peoples R China

年份：2020

卷号：177

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20201008259257);WOS:【SCI-EXPANDED(收录号:WOS:000525303800058)】；

基金：This work was supported by the National Natural Science Foundation of China (81872747, 21672064, 21977082), the National Key R&D Program of China (2017YFB0202600), the Innovative Research Team of High-level Local Universities in Shanghai, the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), Open Funding Project of the State Key Laboratory of Bioreactor Engineering (2018OPEN12), and the Shanghai Sailing Program (17YF1403600).

语种：英文

外文关键词：Curcumin; Polarity-specificity; Polarity fluorescent probe; Lysosome; Live-cell imaging; Design strategy

摘要：Polarity is a crucial characteristic of indispensable organelles, which has been indicated to be closely related to many pathological and biological processes. Hence, a novel design strategy of curcumin-based polarity fluorescent probes was presented. Curcumin attracted much attention due to its environment-sensitivity and optical properties. We reformed the structure of curcumin by attaching a galactose moiety that was used to increase the water solubility and regulate the probe working mode. Furthermore, we also introduced a diethylamino group, which played a substantial role in adjusting the polarity specificity and sensitivity of the probes. We finally screened two polarity-specific fluorescent probes 0301 and 0302. Both probes exhibited remarkable changes in fluorescence from red to green upon increasing polarity of environment. Using 0301 in confocal imaging, we obtained a clear and exact view of lysosomal polarity in different cells, suggesting that the lysosomal polarity in normal cells is higher than that in cancer cells. Also, we use 0301 to monitor variations in lysosomal polarity in live cells induced by dimethylsulfoxide and sucrose, indicating that our probe could specifically monitor the changes of polarity in live cells to elucidate polarity-related biological processes.