文献类型：期刊文献

中文题名：生物基尼龙聚丁内酰胺单体γ-氨基丁酸的纯化工艺研究

英文题名：Study on the Separation and Extraction Technology of Gamma-aminobutyric Acid(GABA)for PA4

作者：赵鹤飞[1];夏泉鸣[1];邱勇隽[1];王耀松[1];蒋丽华[1];赵黎明[1]

机构：[1]华东理工大学,生物反应器工程国家重点实验室,发酵工业分离提取技术研发中心,上海200237

年份：2014

卷号：4

期号：5

起止页码：368

中文期刊名：生物技术进展

外文期刊名：Current Biotechnology

收录：CSTPCD

基金：国家863计划项目(2014AA021202)资助

语种：中文

中文关键词：γ-氨基丁酸;谷氨酸;发酵液;膜分离;离子交换

外文关键词：GABA;glutamic acid;broth;membrane seperation;ion-exchange

摘要：为掌握从谷氨酸发酵液提取生物基尼龙聚丁内酰胺单体γ-氨基丁酸(GABA)的技术,本文研究了膜过滤联合离子交换吸附和洗脱的工艺,并且使用活性炭进行脱色。膜过滤过程中初始大跨膜压差为0.85 bar,过滤温度控制在38℃;离子交换过程采用QY-021-a强酸型阳离子交换树脂,常温下吸附-洗脱的操作方法;经旋转蒸发浓缩后,添加15%(碳对固形物含量之比)活性炭,温度为65℃,搅拌40 min脱色。结果表明:平均膜通量为128 L/m2·h,浓缩倍数为33.3倍。经取样检测膜透过液样品,浓缩液样品计算GABA收率为97.7%,微滤膜以及膜表面污染物上无GABA截留和吸附截留;离子交换经5 BV洗脱后计算收率,吸附-洗脱过程的收率为92.8%;使用活性碳B的脱色率为94.2%,GABA收率为99.2%。该工艺的总收率为85%,该工艺具有工业化应用前景,并且可以通过优化离子交换工艺和设备进一步提高收率。
In order to explore the extraction technology of GABA from fermentation broth with glutamie acid as substrate,membrane filtration associated with ion-exchange adsorption and desorption process were studied in this paper,and followed with decolorization by using active carbon.The initial trans-membrane pressure is 0.85 bar during the filtration process,and the temperature was controlled at 38~C.With QY-021-a strong acid cation exchange resin as packing in ion-exchange column,adsorption-desorption process was exerted at room temperature.After concentrated by rotary evaporation,decolorization was performed using 15%(carbon/solids in the solution)activate carbon,with temperature fixed at 65~C,stirring for 40 min.Results showed that the average flux was 128 L/mz"h,and concentration ratio was 33.3.Samplings to detect membrane permeate showed that the yield of GABA was 97.7%,which indicated that there was no GABA retention of interception and adsorption on microfiltration membrane surface and fouling layer.After collecting 5 BV permeate to calculate the yield of ion-exchange process,results showed that the yield was 92.8%,decolorization ratio was 94.2%by using activate carbon B,and the GABA yield was 99.2%.The total yield of the whole process was up to 85%,which indicated that this process has the prospects for industrial application,and further improvements of yield could be achieved by optimized ion exchange processes and equipment.