文献类型：期刊文献

英文题名：A Polymer-Based Matrix for Effective SALDI Analysis of Lipids

作者：Yu, Jing[1,2,3];Kang, Yan[1,2,3];Zhang, Hongyang[4];Yang, Feng[5];Zhen, Huajun[6];Zhu, Xixi[1,2,3];Wu, Ting[1,2,3];Du, Yiping[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn & Shanghai, Shanghai 200237, Peoples R China;[5]Fudan Univ, Dept Lab Med, Huadong Hosp, Shanghai, Peoples R China;[6]East China Univ Sci & Technol, Sch Resources & Environm Engn, Shanghai, Peoples R China

年份：2021

卷号：32

期号：5

起止页码：1189

外文期刊名：JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

收录：;EI(收录号：20212210415447);WOS:【SCI-EXPANDED(收录号:WOS:000648704200007)】；

基金：This work was supported by Science and Technology Commission of Shanghai Municipality (No. 19142201200) and Key Laboratory of Cigarette Smoke Research, CNTC (Shanghai Tobacco Group Co., Ltd.). We thank Dr. Dong Jiang of Research Center of Analysis and Test in East China University of Science and Technology for his support in XPS characterization and analysis.

语种：英文

外文关键词：polymer-based matrix; PVDF modification; SALDI MS; lipids

摘要：Surface-assisted laser desorption/ionization (SALDI) has become an attractive branch of matrix-assisted laser desorption/ionization (MALDI) and has been successfully applied for the detection of small molecules due to the lack of the interference of matrix. Herein, the low-cost and highly accessible polyvinylidene fluoride (PVDF) was modified using a facile alkali treatment and investigated as a SALDI matrix. The modified PVDF has a strong optical absorption and can be applied as a dual-mode substrate for both SALDI MS and SALDI imaging analysis. Modified PVDF powder showed superior performance in SALDI MS analysis of lipids, with good reproducibility, high sensitivity, and low background interference, especially for triacylglycerols (TAGs) and fatty acids. Additionally, the lipids in raw and extracted serum were both successfully determined with modified PVDF powder. A modified PVDF membrane (m-PVDF-m) showed excellent ability in lipids imaging in tissues due to its flat surface, mass signal enhancement, and elimination of matrix coating. The distribution of several TAGs and cholesteryl esters on mouse kidney section was presented by SALDI imaging directly on m-PVDF-m. These results demonstrated that modified PVDF materials presented exciting opportunities as matrices for the first time in SALDI MS acquisition and SALDI imaging.