文献类型：期刊文献

英文题名：Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae

作者：Zheng, Yu[1];Zhao, Li[1];Zhang, Jianguo[1];Zhang, Haiyi[1];Ma, Xingyuan[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：105

期号：5

起止页码：508

外文期刊名：JOURNAL OF BIOSCIENCE AND BIOENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000257930000011)】；

语种：英文

外文关键词：1,3-propanediol; glycerol; glycerol-3-phosphatase; glycerol-3-phosphate dehydrogenase; Klebsiella pneumoniae

摘要：As a valuable chemical, 1,3-propanediol (1,3-PD) could be biosynthesized by glycerol fermentation. However, no natural microorganisms that could directly convert glucose into 1,3-PD have been found so far. In this work, genes coding for two enzymes, glycerol-3-phosphate dehydrogenase (GPD, EC 1.1.1.8) and glycerol-3-phosphatase (GPP, EC 3.1.3.21), which were responsible for glycerol production, were organized into the plasmid pUC18K under control of the respective lac promoters. Two recombinant proteins were expressed successfully in wild-type Klebsiella pneumoniae. A glycerol concentration of 6.8 g l(-1) was obtained in flask culture. When glucose was exhausted, dihydroxyacetone was added and medium pH was adjusted to 7.0, and then a 1,3-PD concentration of 0.58 g l(-1) was achieved with engineered K. pneumoniae from glucose.