文献类型：期刊文献

英文题名：Bioconcentration and metabolism of BDE-209 in the presence of titanium dioxide nanoparticles and impact on the thyroid endocrine system and neuronal development in zebrafish larvae

作者：Wang, Qiangwei[1,2];Chen, Qi[1,2];Zhou, Peng[3];Li, Wenwen[4];Wang, Junxia[3];Huang, Changjiang[4];Wang, Xianfeng[1];Lin, Kuangfei[3];Zhou, Bingsheng[1]

机构：[1]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China;[2]Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;[3]E China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[4]Wenzhou Med Univ, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China

年份：2014

卷号：8

起止页码：196

外文期刊名：NANOTOXICOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000340524300020)】；

基金：The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article. This work was supported by grants from the National Natural Science Foundation of China (No. 21237005), the National Environmental Protection Public Welfare Science and Technology Research Program of China (No. 201309047) and the State key Laboratory of Freshwater Ecology and Biotechnology (No. 2011FBZ13).

语种：英文

外文关键词：BDE-209; bioconcentration and metabolism; co-exposure; developmental neurotoxicity; titanium dioxide nanoparticles; thyroid endocrine disruption; zebrafish larvae

摘要：Interactions between organic toxicants and nanoparticles (NPs) in the aquatic environment may modify toxicant bioavailability and consequently the toxicant's environmental fate and toxicity. Therefore, we investigated the influence of titanium dioxide NPs (nano-TiO2) on deca-BDE (BDE-209; a polybrominated diphenyl ether congener) bioconcentration, metabolism and its effects on the thyroid endocrine system in zebrafish (Danio rerio) larvae. Zebrafish embryos were exposed to various concentrations of BDE-209 alone or in combination with nano-TiO2 (0.1 mg/L) until 7-day post-fertilization. Nano-TiO2 can adsorb BDE-209 and nano-TiO2 is taken up into developing zebrafish larvae. Chemical measurements showed that BDE-209 was bioconcentrated and metabolized in zebrafish larvae, and BDE-209 uptake was enhanced by nano-TiO2. Furthermore, increased BDE-209 metabolites were detected in larvae co-exposed with nano-TiO2. BDE-209 exposure significantly increased whole-body thyroid hormone contents (T-3 and T-4); T-4 content significantly increased in the larvae co-exposed with nano-TiO2. Nano-TiO2 exposure alone did not induce generation of reactive oxygen species, lipid peroxidative oxidation, gene transcription or thyroid hormone levels. Upregulation of several gene transcriptions (tsh beta, tg, diO(2)) in the hypothalamic-pituitary-thyroid axis was also observed. Furthermore, co-exposure of nano-TiO2 and BDE-209 caused a decrease in locomotion activity and downregulation of specific genes and proteins involved in the central nervous system of developing zebrafish larvae (e.g. myelin basic protein and alpha 1-tubulin). These results indicate nano-TiO2 enhances BDE-209 bioavailability and metabolism, leading to thyroid endocrine disruption and developmental neurotoxicity in zebrafish.